Wan Nazaimoon W M, Satgunasingam N, Khalid B
Radiochemistry Division, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur.
Malays J Pathol. 1990 Jun;12(1):13-20.
A simple and sensitive double-antibody radioimmunoassay for human growth hormone (HGH) was developed, optimised and validated. The anti-hGH sera raised in 2 rabbits were highly specific with low cross-reactions of 0.19% and 0.3% with human placental lactogen and 0.21% and 0.13% with human prolactin. The mean sensitivity of the assay determined from 28 assays was found to be 0.4 +/- 0.2 mIU/L. Mean recovery of added exogenous hGH was 98.8 +/- 6.8%. Linearity studies of samples diluted at 1:2, 1:4 and 1:8 gave values of 101.3 +/- 5.3%, 109.6 +/- 13.4% and 97.3 +/- 13% respectively of those expected. The reproducibility of the assay was good; within assay coefficient of variation for serum samples with GH concentrations of 2.7, 13.6 and 28.2 mU/l ranged from 5.1 to 8.3% while the inter-assay precision varied from 4.9 to 10.3%. The in-house assay showed good correlation (r = 0.96, p less than 0.001) with a commercial HGH RIA kit (Dainabot, Japan). A reference normal adult fasting GH level of less than 7 mIU/l was established from 95 samples assayed by this method.
开发、优化并验证了一种用于检测人生长激素（HGH）的简单且灵敏的双抗体放射免疫分析方法。在2只兔子体内产生的抗hGH血清具有高度特异性，与人胎盘催乳素的交叉反应率较低，分别为0.19%和0.3%，与人催乳素的交叉反应率分别为0.21%和0.13%。通过28次检测确定该分析方法的平均灵敏度为0.4±0.2 mIU/L。添加的外源性hGH的平均回收率为98.8±6.8%。对按1:2、1:4和1:8稀释的样品进行线性研究，得到的值分别为预期值的101.3±5.3%、109.6±13.4%和97.3±13%。该分析方法的重复性良好；对于生长激素浓度分别为2.7、13.6和28.2 mU/l的血清样品，批内变异系数在5.1%至8.3%之间，而批间精密度在4.9%至10.3%之间。内部分析方法与商用HGH放射免疫分析试剂盒（日本Dainabot公司）显示出良好的相关性（r = 0.96，p＜0.001）。通过该方法对95份样品进行检测，确定正常成人空腹生长激素水平的参考值低于7 mIU/l。
