Sweeney D, Beeley J A
Oral Biochemistry Unit (Oral Biology Group), University of Glasgow Dental Hospital and School, Scotland.
Arch Oral Biol. 1990;35(9):741-6. doi: 10.1016/0003-9969(90)90097-t.
An ELISA method has been developed for the determination of albumin in human parotid saliva. The range of sensitivity is 2-80 micrograms/l and it is suitable for use with volumes of 5 microliters or less of freshly collected parotid fluid, without prior sample preparation. Calibration data were fitted by microcomputer to an exponential function. The albumin concentration of parotid saliva was 1.9 +/- 1.53 mg/l, but levels fell on sample storage. Isoelectric focusing, followed by immunoblotting with antiserum to human serum albumin, showed identical double bands of pI 4.9-5.0 in albumin from both serum and saliva, thereby confirming that the antigens from the two sources are identical.
已开发出一种酶联免疫吸附测定（ELISA）方法用于测定人腮腺唾液中的白蛋白。灵敏度范围为2 - 80微克/升，适用于5微升或更少的新鲜采集腮腺液，无需预先进行样品制备。校准数据由微型计算机拟合为指数函数。腮腺唾液中白蛋白浓度为1.9±1.53毫克/升，但样品储存时浓度会下降。等电聚焦，随后用抗人血清白蛋白抗血清进行免疫印迹，结果显示血清和唾液中的白蛋白均有相同的pI 4.9 - 5.0双条带，从而证实两种来源的抗原是相同的。
