Saad A Y, Gartner L P, Hiatt J L
Department of Anatomy Dental School, University of Maryland, Baltimore 21201.
Biol Struct Morphog. 1990;3(1):31-5.
Fetuses of pregnant CD-1 mice, exposed to intraperitoneal injection of 0.1% nicotine sulfate at a dose of 1.67 mg/kg body weight/day on gestational days 6-15, were compared with control (saline injected and non-injected) fetuses to assess the effects of nicotine on fetal growth in general and palatogenesis in particular. A total of 59 pregnant females (18 experimental and 41 control) were sacrificed on the 18 th gestational day and their fetuses were examined gross morphologically and histologically (using serial sections through the head in the frontal plane). Data analysis revealed that maternal weight gain, crown-rump length, fetal weight and head dimensions were significantly reduced in nicoted treated animals when compared to those of the controls. Histological examination revealed that 9.6% of fetuses of nicotine injected mothers presented clefts of the palate, whereas none of the control fetuses had that anomaly. It was concluded that nicotine has a detrimental effect on general growth and development as well as on palatogenesis of mice.
将怀孕的CD-1小鼠在妊娠第6至15天腹腔注射剂量为1.67毫克/千克体重/天的0.1%硫酸尼古丁，将其胎儿与对照（注射生理盐水和未注射的）胎儿进行比较，以评估尼古丁对胎儿总体生长尤其是腭发育的影响。在妊娠第18天，共处死59只怀孕雌性小鼠（18只实验小鼠和41只对照小鼠），并对其胎儿进行大体形态学和组织学检查（使用额面头部连续切片）。数据分析显示，与对照组相比，经尼古丁处理的动物母体体重增加、顶臀长度、胎儿体重和头部尺寸均显著降低。组织学检查显示，注射尼古丁的母亲所生胎儿中有9.6%出现腭裂，而对照胎儿均无此异常。得出的结论是，尼古丁对小鼠的总体生长发育以及腭发育有不利影响。
