Hoekzema R, Hwan S B, Rothova A, van Haren M A, Donoso L A, Kijlstra A
Department of Ophthalmo-Immunology, Netherlands Ophthalmic Research Institute, Amsterdam.
Curr Eye Res. 1990 Dec;9(12):1177-83. doi: 10.3109/02713689009003474.
Interphotoreceptor retinoid binding protein (IRBP) is a 136,000 molecular weight photoreceptor cell protein capable of inducing an experimental autoimmune uveitis (EAU) in susceptible animal strains. The occurrence of serum antibodies against human (Hu) or bovine (Bo) IRBP was investigated in patients with uveitis and healthy controls. A sensitive ELISA detected anti-IRBP in approximately 50% of patients and controls, without apparent differences in the mean level, titre or avidity and irrespective of the origin of the antigen. Although the correlation (p less than 0.001) between anti-HuIRBP and anti-BoIRBP levels in uveitis sera suggested the presence of crossreacting antibodies, these sera also contained antibodies specific for either the human or the bovine antigen. The only difference between patients and controls was the greater ability of antibodies in uveitis sera (p less than 0.05) to recognize a synthetic peptide of HuIRBP, which induces severe EAU in rats. We conclude that autoantibodies to IRBP occur naturally in man and are not increased in patients with uveitis.
光感受器间类视黄醇结合蛋白（IRBP）是一种分子量为136,000的光感受器细胞蛋白，能够在易感动物品系中诱发实验性自身免疫性葡萄膜炎（EAU）。我们在葡萄膜炎患者和健康对照中研究了抗人（Hu）或抗牛（Bo）IRBP血清抗体的出现情况。一种灵敏的酶联免疫吸附测定法（ELISA）在大约50%的患者和对照中检测到了抗IRBP，在平均水平、滴度或亲和力方面无明显差异，且与抗原来源无关。尽管葡萄膜炎血清中抗HuIRBP和抗BoIRBP水平之间的相关性（p小于0.001）提示存在交叉反应抗体，但这些血清中也含有针对人或牛抗原的特异性抗体。患者和对照之间的唯一差异在于葡萄膜炎血清中的抗体（p小于0.05）识别HuIRBP合成肽的能力更强，该合成肽可在大鼠中诱发严重的EAU。我们得出结论，抗IRBP自身抗体在人类中自然存在，在葡萄膜炎患者中并未增加。
