Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Ave., P.O. Box 250509, Charleston, South Carolina 29425, USA.
Adv Exp Med Biol. 2010;688:46-59. doi: 10.1007/978-1-4419-6741-1_3.
Sphingolipid (SPL) metabolism (Fig. 1) serves a key role in the complex mechanisms regulating cellular stress responses to environment. Several SPL metabolites, especially ceramide (Cer), sphingosine (Sph) and sphingosinel-phosphate (S1P) act as key bioactive molecules governing cell growth and programmed cell death (Fig. 2). Perturbations in sphingolipids of one type may enhance or interfere with the action of another. To monitor changes in SPL composition therefore, reliable analytical methods are necessary. Here we present the liquid chromatography tandem mass spectrometry (LC-MS/MS) approach for simultaneous qualitative and quantitative monitoring of SPL components (classes and molecular species) in biological material as an effective tool to study sphingolipid signaling events. The LC-MS/MS methodology is the only available technique that provides high specificity and sensitivity, along with a wealth of structural identification information.
鞘脂代谢（图 1）在调节细胞对应激环境反应的复杂机制中起着关键作用。几种鞘脂代谢物，特别是神经酰胺（Cer）、鞘氨醇（Sph）和鞘氨醇-1-磷酸（S1P），作为调节细胞生长和程序性细胞死亡的关键生物活性分子（图 2）。一种鞘脂的紊乱可能会增强或干扰另一种鞘脂的作用。因此，为了监测鞘脂组成的变化，需要可靠的分析方法。在这里，我们提出了一种液相色谱-串联质谱（LC-MS/MS）方法，用于同时定性和定量监测生物材料中的鞘脂成分（类别和分子种类），这是研究鞘脂信号事件的有效工具。LC-MS/MS 方法是唯一能够提供高特异性和灵敏度以及丰富结构鉴定信息的技术。
