Department of Chemistry, Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QR, UK.
Chem Commun (Camb). 2010 Nov 28;46(44):8463-5. doi: 10.1039/c0cc03292a. Epub 2010 Oct 5.
During catalysis by hydrogenases, entities no larger than H(2) or H(+) reach and leave a deeply buried active site, by as yet unidentified pathways. Novel experiments, conducted mainly with the membrane-bound [NiFe]-hydrogenase from Ralstonia eutropha, explore why small excess gas pressures (H(2) or He) attenuate the rate of H(2) oxidation.
在氢化酶的催化过程中，比 H(2)或 H(+)大不了多少的物质通过目前尚未确定的途径到达并离开深埋的活性部位。新型实验主要使用来自 Ralstonia eutropha 的膜结合 [NiFe]-氢化酶进行，探索了为什么小的过压气体（H(2)或 He）会降低 H(2)氧化的速率。
