Zhou Zhihua, Yi Qingfeng, Gao Yongyi, Liu Qingquan, Liu Lihua, Zeng Wennan, Liu Xiaoping
Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, PR China.
J Med Eng Technol. 2010 Oct-Nov;34(7-8):455-61. doi: 10.3109/03091902.2010.519813. Epub 2010 Oct 6.
The effects of metal ions on the cell DNA and RNA synthetic functions and the destruction of cell defensive system caused by metal ions were studied on the investigation of the effects of metal ions on cell alkaline phosphatase (ALP) activity and cell metabolism ability. The poisonous behaviours of metal ions [Cr (VI), Ni, V, Cr (III), Ag, and Al] on living tissue were both quantitatively and qualitatively measured. It was shown that Cr (VI) has a notable action on both cellular DNA and RNA synthetic functions, and following that, were Ni, V. The limitation action of Cr (III) on the synthetic abilities of DNA and RNA increased with the increase of Cr (III) concentration. Different to other five metal ions, the limitation of Al ion on RNA was greater than that on DNA. The limitation of V, Ag ions on DNA was same to that on RNA. Trace Cr (VI), in culture medium resulted in the decreasing of GSH in living tissue. Similar to that, a little higher Ni ion concentration seriously reduced the GSH content in living tissue. It was suggested that Cr (VI), Ni, might destroy and/or disturb the orientation of the microtubulin in cell skeleton. For other four metal ions, together with increasing the concentration in culture medium, the osmotic pressure increased. Consequently, more metal ions entered cell membrane, more GSH were lost in cell.
在研究金属离子对细胞碱性磷酸酶（ALP）活性和细胞代谢能力影响的基础上，研究了金属离子对细胞DNA和RNA合成功能的影响以及金属离子对细胞防御系统的破坏作用。对金属离子[Cr（VI）、Ni、V、Cr（III）、Ag和Al]对活组织的毒害行为进行了定量和定性测定。结果表明，Cr（VI）对细胞DNA和RNA合成功能均有显著作用，其次是Ni、V。Cr（III）对DNA和RNA合成能力的限制作用随Cr（III）浓度的增加而增强。与其他五种金属离子不同，Al离子对RNA的限制作用大于对DNA的限制作用。V、Ag离子对DNA的限制作用与对RNA的限制作用相同。培养基中的微量Cr（VI）导致活组织中谷胱甘肽（GSH）含量降低。同样，稍高浓度的Ni离子会严重降低活组织中GSH的含量。有人认为，Cr（VI）、Ni可能破坏和/或干扰细胞骨架中微管蛋白的取向。对于其他四种金属离子，随着培养基中浓度的增加，渗透压升高。因此，更多的金属离子进入细胞膜，细胞内更多的GSH流失。
