Wu Xue-qing, Wan Hua, Li Xin-rong
Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Jul;30(7):717-9, 756.
To explore the immune response induced by HER2/neu oncogene in the breast cancer (BC) carcinogenesis process and the immunological mechanism of Ru'ai Shuhou Recipe (RSR) in the prevention and treatment of BC.
HER2/neu transgenic spontaneous breast tumor model mice were fed with RSR from 5 weeks old, the occurrence of breast tumor in them was observed, and the changes of T cell-mediated immune response and associated cytokines were detected during the carcinogenesis process, i. e., when mice aged between 15 and 25 weeks.
RSR showed significant effects in postponing and reducing the carcinogenesis of primary breast tumor, up-regulating the amount of T cell in splenic lymphocyte in tumor-bearing mice, promoting the proliferation of T lymphocyte, and inducing the secretion of cytokines such as interleukin-2, interleukin-12 and interferon-y.
A serial immune response reveals in the carcinogenesis process. The immunologic function of HER-2/neu transgenic mice is significantly different to that of the same strain non-transgenic mice. Effect of RSR in preventing and postponing breast cancer carcinogenesis is possibly realized through enhancing the anti-tumor immune response of transgenic mice themselves.
探讨HER2/neu癌基因在乳腺癌致癌过程中诱导的免疫反应以及乳癌术后方在预防和治疗乳腺癌中的免疫机制。
HER2/neu转基因自发性乳腺肿瘤模型小鼠从5周龄开始用乳癌术后方喂养，观察其乳腺肿瘤的发生情况，并在致癌过程中，即小鼠15至25周龄时，检测T细胞介导的免疫反应及相关细胞因子的变化。
乳癌术后方在延缓和减少原发性乳腺肿瘤致癌方面显示出显著效果，上调荷瘤小鼠脾淋巴细胞中T细胞数量，促进T淋巴细胞增殖，并诱导白细胞介素-2、白细胞介素-12和干扰素-γ等细胞因子的分泌。
致癌过程中呈现出一系列免疫反应。HER-2/neu转基因小鼠的免疫功能与同品系非转基因小鼠显著不同。乳癌术后方预防和延缓乳腺癌致癌的作用可能是通过增强转基因小鼠自身的抗肿瘤免疫反应来实现的。
