Wolff R L, Sebedio J L, Grandgirard A
ISTAB-ITERG, Laboratoire de Lipochimie Alimentaire, Universite de Bordeaux I, Talence, France.
Lipids. 1990 Dec;25(12):859-62. doi: 10.1007/BF02535910.
The use of a capillary column coated with 100% cyanopropyl polysiloxane (CP(TM)Sil 88) allows the separation of several fatty acids associated with fat deficiency. Starting with liver mitochondrial phospholipids of weanling rats fed a fat-free diet, an unusual fatty acid was isolated, along with 20:4n-6, by thin-layer chromatography on AgNO3-impregnated silica gel plates. After partial hydrazine reduction of these acids, the resulting monoenes were isolated and subjected to ozonolysis in BF3/methanol. The resulting monomethyl and dimethyl esters were identified by gas chromatography/mass spectrometry. Our data indicate that the unusual component corresponds to 20:4n-7. Based on published biochemical and analytical studies and on our own chromatographic retention data, some of the other unusual fatty acids were tentatively identified as 18:2n-7, 20:2n-7 and 20:3n-7. The CP(TM)Sil 88 column appears to be a simple and useful tool for the separation of fatty acids of the palmitoleate series.
使用涂覆有100%氰丙基聚硅氧烷（CP(TM)Sil 88）的毛细管柱可分离几种与脂肪缺乏相关的脂肪酸。以喂食无脂饮食的断奶大鼠的肝脏线粒体磷脂为起始材料，通过在硝酸银浸渍的硅胶板上进行薄层色谱，分离出一种不寻常的脂肪酸以及20:4n-6。在对这些酸进行部分肼还原后，分离得到生成的单烯，并在BF3/甲醇中进行臭氧分解。通过气相色谱/质谱法鉴定生成的单甲酯和二甲酯。我们的数据表明，这种不寻常的成分对应于20:4n-7。基于已发表的生化和分析研究以及我们自己的色谱保留数据，一些其他不寻常的脂肪酸被初步鉴定为18:2n-7、20:2n-7和20:3n-7。CP(TM)Sil 88柱似乎是分离棕榈油酸系列脂肪酸的一种简单且有用的工具。
