Al Makdessi S, Andrieu J L, Bacconin A, Fugier J C, Herilier H, Faucon G
J Chromatogr. 1985 Apr 12;339(1):25-34. doi: 10.1016/s0378-4347(00)84624-1.
The fatty acids of three lipid classes (free fatty acids, triglycerides, and cholesteryl esters) from dog heart were analysed by gas chromatography. Samples of the left ventricle were homogenized and total lipids were extracted. After separation by thin-layer chromatography, the bands of the lipid classes studied were scraped off, transmethylated according to the boron trifluoride-methanol procedure, and the fatty acid methyl esters were extracted and analysed. The problems related to the quantitation of fatty acids were investigated, namely transmethylation procedure, thin-layer chromatography, and gas chromatographic conditions. Fatty acid methyl esters were separated on capillary columns coated in the laboratory with SP 2340 stationary phase. The high performance of the separation ensured the reliability and the precision of the analysis.
采用气相色谱法分析了犬心脏中三类脂质（游离脂肪酸、甘油三酯和胆固醇酯）的脂肪酸。取左心室样本进行匀浆，提取总脂质。经薄层色谱分离后，刮下所研究脂质类别的条带，按照三氟化硼 - 甲醇法进行甲基化处理，然后提取并分析脂肪酸甲酯。研究了与脂肪酸定量相关的问题，即甲基化方法、薄层色谱和气相色谱条件。脂肪酸甲酯在实验室涂覆有SP 2340固定相的毛细管柱上进行分离。高效的分离确保了分析的可靠性和精密度。
