Yang Yong, Dai Yumei, Chen Zhenhua, Qin Zhongjun
Key Laboratory of Synthetic Biology, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Wei Sheng Wu Xue Bao. 2010 Aug;50(8):1008-13.
Streptomyces strains were isolated from soil samples of Tibet, five small linear plasmids were detected by pulsed-field gel electrophoresis.
Cloning, sequencing and analysis of telomeres of these plasmids.
The telomeres were cloned by a modified procedure--"alkaline treatment and enzyme digestion in gels".
Telomeres of five linear plasmids were cloned and sequenced. Compared with the typical Streptomyces telomeres, the newly identified telomeres contained multiple palindromes, but some could not "fold-back" of their first conserved palindrome I with the internal palindromes to form a "super-hairpin", and palindromes of some telomeres did not contain the 3-nt "loop".
New telomere sequences were cloned by a modified procedure. Both folding-back of the telomere palindromes and 3-nt loop of palindromes varied among telomeres.
从西藏土壤样本中分离链霉菌菌株，通过脉冲场凝胶电泳检测到5个小的线性质粒。
对这些质粒的端粒进行克隆、测序及分析。
采用改良的“凝胶内碱处理及酶切”方法克隆端粒。
克隆并测序了5个线性质粒的端粒。与典型的链霉菌端粒相比，新鉴定的端粒含有多个回文序列，但有些不能将其第一个保守回文序列I与内部回文序列“回折”形成“超级发夹”，且有些端粒的回文序列不包含3个核苷酸的“环”。
通过改良方法克隆到了新的端粒序列。端粒回文序列的回折及回文序列的3个核苷酸环在不同端粒间存在差异。
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