一种用于 PCR 测序链霉菌和分枝杆菌线性复制子端粒的自连接方法。
A self-ligation method for PCR-sequencing the telomeres of Streptomyces and Mycobacterium linear replicons.
机构信息
Key laboratory of Synthetic Biology, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, People's Republic of China.
出版信息
J Microbiol Methods. 2012 Aug;90(2):105-7. doi: 10.1016/j.mimet.2012.04.012. Epub 2012 Apr 26.
Actinomycete species from many genera often harbor linear plasmids and some contain linear chromosomes. A self-ligation and PCR-sequencing method was developed for identifying three novel telomere sequences of linear plasmids of Streptomyces and Mycobacterium. This and four previously described methods for actinomycetes telomere cloning and sequencing are discussed.
放线菌属的许多种经常含有线性质粒，有些则含有线性染色体。本文建立了一种用于鉴定链霉菌属和分枝杆菌属线性质粒的三个新端粒序列的自连接和 PCR 测序方法。本文讨论了这种方法以及之前描述的用于放线菌端粒克隆和测序的四种方法。
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