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[大鼠嗅觉神经上皮超分子组织的旋转铂 - 碳阴影投射冷冻蚀刻分析]

[An analysis of the supramolecular organization of the olfactory neuroepithelium in the rat by freeze etching with rotary platinum-carbon shadow-casting].

作者信息

Popov V I, Khutsian S S, Allakhverdov B L, Novikov Iu V, Novoselov V I, Fesenko E E

出版信息

Tsitologiia. 1990;32(11):1090-3.

PMID:2093245
Abstract

The rat olfactory epithelium was analysed by freeze-deep etching and Pt/C rotary replication. Ultrathin sections and freeze-etching findings of proximal (dendrite) and distal (axon) parts of bipolar olfactory neurons are examined. The supramolecular organization of neuron membranes and intracellular cytoskeleton structure is studied. The role of the Schwann cells in formation of isolated axon bundles is discussed. Methods of the whole neuroepithelium preparation for freeze-etching and different easy approaches of obtaining the Pt/C rotary shadowing replicas with high resolution (15-20A) are presented.

摘要

通过冷冻深度蚀刻和铂/碳旋转复型技术对大鼠嗅觉上皮进行分析。检查双极嗅觉神经元近端(树突)和远端(轴突)部分的超薄切片及冷冻蚀刻结果。研究神经元膜的超分子组织和细胞内细胞骨架结构。讨论施万细胞在分离轴突束形成中的作用。介绍了用于冷冻蚀刻的整个神经上皮制备方法以及获得高分辨率(15 - 20埃)铂/碳旋转阴影复制品的不同简便方法。

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