College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
Toxicol In Vitro. 2011 Feb;25(1):110-6. doi: 10.1016/j.tiv.2010.09.014. Epub 2010 Oct 27.
The chemoprotective effect of N-acetylcysteine (NAC), a sulfhydryl-containing antioxidant, on nano titanium dioxide (nano-TiO(2)) induced oxidative stress and apoptosis in human keratinocyte (HaCaT) cells was assessed. HaCaT cells were pretreated with NAC followed by treatment with 200 μg/ml nano-TiO(2), then exposed to ultraviolet A (UVA, 365 nm) for 1 h and cultured for 24 h. Intracellular reactive oxygen species (ROS) and nitric oxide (NO) formation, mitochondrial membrane potential (MMP), apoptosis and the content of the lipid peroxidation product malondialdehyde (MDA) were measured. Keratin 6 (K6) mRNA expression was also analyzed. The results showed that NAC strongly inhibited ROS and NO production in nano-TiO(2) treated cells. The extent of lipid peroxidation was also decreased in the presence of NAC. In addition, NAC suppressed nano-TiO(2) induced apoptosis and increased K6 mRNA expression. The results indicated that NAC could prevent oxidative stress and apoptosis induced by nano-TiO(2) in HaCaT cells.
评估了含巯基抗氧化剂 N-乙酰半胱氨酸 (NAC) 对纳米二氧化钛 (nano-TiO(2)) 诱导的人角质形成细胞 (HaCaT) 细胞氧化应激和细胞凋亡的化学保护作用。HaCaT 细胞先用 NAC 预处理，然后用 200 μg/ml 的纳米 TiO(2)处理，然后用紫外线 A (UVA，365nm) 照射 1 小时，培养 24 小时。测量细胞内活性氧 (ROS) 和一氧化氮 (NO) 的形成、线粒体膜电位 (MMP)、凋亡和脂质过氧化产物丙二醛 (MDA) 的含量。还分析了角蛋白 6 (K6) mRNA 的表达。结果表明，NAC 强烈抑制纳米 TiO(2)处理细胞中 ROS 和 NO 的产生。NAC 的存在也降低了脂质过氧化的程度。此外，NAC 抑制了纳米 TiO(2)诱导的细胞凋亡并增加了 K6 mRNA 的表达。结果表明，NAC 可以防止纳米 TiO(2)诱导的 HaCaT 细胞氧化应激和细胞凋亡。
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