Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China.
Toxins (Basel). 2018 Oct 9;10(10):407. doi: 10.3390/toxins10100407.
Zearalenone (ZEN), a nonsteroidal estrogen mycotoxin, is widely found in feed and foodstuffs. Intestinal cells may become the primary target of toxin attack after ingesting food containing ZEN. Porcine small intestinal epithelial (SIEC02) cells were selected to assess the effect of ZEN exposure on the intestine. Cells were exposed to ZEN (20 µg/mL) or pretreated with (81, 162, and 324 µg/mL) N-acetylcysteine (NAC) prior to ZEN treatment. Results indicated that the activities of glutathione peroxidase (Gpx) and glutathione reductase (GR) were reduced by ZEN, which induced reactive oxygen species (ROS) and malondialdehyde (MDA) production. Moreover, these activities increased apoptosis and mitochondrial membrane potential (ΔΨm), and regulated the messenger RNA (mRNA) expression of Bax, Bcl-2, caspase-3, caspase-9, and cytochrome c (cyto c). Additionally, NAC pretreatment reduced the oxidative damage and inhibited the apoptosis induced by ZEN. It can be concluded that ZEN-induced oxidative stress and damage may further induce mitochondrial apoptosis, and pretreatment of NAC can degrade this damage to some extent.
玉米赤霉烯酮（ZEN）是一种非甾体类雌激素真菌毒素，广泛存在于饲料和食品中。摄入含 ZEN 的食物后，肠道细胞可能成为毒素攻击的主要目标。选择猪小肠上皮（SIEC02）细胞来评估 ZEN 暴露对肠道的影响。细胞先经 ZEN（20μg/mL）或 N-乙酰半胱氨酸（NAC，81、162 和 324μg/mL）预处理，然后再用 ZEN 处理。结果表明，ZEN 降低了谷胱甘肽过氧化物酶（Gpx）和谷胱甘肽还原酶（GR）的活性，诱导活性氧（ROS）和丙二醛（MDA）的产生。此外，这些活性增加了细胞凋亡和线粒体膜电位（ΔΨm），并调节 Bax、Bcl-2、caspase-3、caspase-9 和细胞色素 c（cyto c）的信使 RNA（mRNA）表达。此外，NAC 预处理减少了 ZEN 诱导的氧化损伤并抑制了细胞凋亡。由此可以得出结论，ZEN 诱导的氧化应激和损伤可能进一步诱导线粒体凋亡，NAC 的预处理在一定程度上可以降解这种损伤。
