Department of Chemistry, Northeastern University, Shenyang 110819, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Dec;77(5):1115-21. doi: 10.1016/j.saa.2010.09.012. Epub 2010 Sep 17.
The eight-coordinate (enH2)YIII(pdta)(H2O)·10H2O (en=ethylenediamine and H4pdta=1,3-propylenediamine-N,N,N',N'-tetraacetic acid) was synthesized, meanwhile its molecular and crystal structures were determined by single-crystal X-ray diffraction technology. The interaction between [Y(III)(pdta)(H2O)]2(2-) and bovine serum albumin (BSA) was investigated by UV-vis and fluorescence spectra. The results indicate that [YIII(pdta)(H2O)]2(2-) quenched effectively the intrinsic fluorescence of BSA via a static quenching process with the binding constant (Ka) of the order of 10(4). Meanwhile, the binding and damaging sites to BSA molecules were also estimated by synchronous fluorescence. Results indicate that the hydrophobic environments around Trp and Tyr residues were all slightly changed. The thermodynamic parameters (ΔG=-25.20 kJ mol(-1), ΔH=-26.57 kJ mol(-1) and ΔS=-4.58 J mol(-1) K(-1)) showed that the reaction was spontaneous and exothermic. What is more, both ΔH and ΔS were negative values indicated that hydrogen bond and Van der Waals forces were the predominant intermolecular forces between [YIII(pdta)(H2O)]2(2-) and BSA.
八配位（enH2）[YIII（pdta）（H2O）]（2）·10H2O（en=乙二胺，H4pdta=1,3-丙二胺-N,N,N',N'-四乙酸）的合成及晶体结构
八配位（enH2）[YIII（pdta）（H2O）]（2）·10H2O（en=乙二胺，H4pdta=1,3-丙二胺-N,N,N',N'-四乙酸）的合成及晶体结构
用单晶 X 射线衍射技术对其分子和晶体结构进行了测定。用紫外可见光谱和荧光光谱研究了[Y（III）（pdta）（H2O）]2（2-）与牛血清白蛋白（BSA）之间的相互作用。结果表明，[YIII（pdta）（H2O）]2（2-）通过静态猝灭过程有效猝灭了 BSA 的固有荧光，其结合常数（Ka）为 10（4）级。同时，通过同步荧光也估算了与 BSA 分子的结合和破坏部位。结果表明，色氨酸和酪氨酸残基周围的疏水环境都略有变化。热力学参数（ΔG=-25.20 kJ mol-1，ΔH=-26.57 kJ mol-1，ΔS=-4.58 J mol-1 K-1）表明该反应是自发的和放热的。更重要的是，ΔH 和 ΔS 均为负值表明，[YIII（pdta）（H2O）]2（2-）与 BSA 之间主要的分子间作用力是氢键和范德华力。
