Díaz-Valdés Nancy, Comendador Miguel A, Sierra L María
Área de Genética, Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias (IUOPA), University of Oviedo, 33006 Oviedo, Spain.
J Nucleic Acids. 2010 Sep 23;2010:416364. doi: 10.4061/2010/416364.
The D. melanogaster mus308 gene, highly conserved among higher eukaryotes, is implicated in the repair of cross-links and of O-ethylpyrimidine DNA damage, working in a DNA damage tolerance mechanism. However, despite its relevance, its possible role on the processing of different DNA ethylation damages is not clear. To obtain data on mutation frequency and on mutation spectra in mus308 deficient (mus308(-)) conditions, the ethylating agent diethyl sulfate (DES) was analysed in postmeiotic male germ cells. These data were compared with those corresponding to mus308 efficient conditions. Our results indicate that Mus308 is necessary for the processing of oxygen and N-ethylation damage, for the survival of fertilized eggs depending on the level of induced DNA damage, and for an influence of the DNA damage neighbouring sequence. These results support the role of mus308 in a tolerance mechanism linked to a translesion synthesis pathway and also to the alternative end-joinig system.
黑腹果蝇的mus308基因在高等真核生物中高度保守，参与交联修复和O-乙基嘧啶DNA损伤修复，在DNA损伤耐受机制中发挥作用。然而，尽管其具有相关性，但其在不同DNA乙基化损伤处理中的可能作用尚不清楚。为了获得mus308缺陷（mus308(-)）条件下的突变频率和突变谱数据，在减数分裂后雄性生殖细胞中分析了乙基化剂硫酸二乙酯（DES）。将这些数据与mus308功能正常条件下的数据进行比较。我们的结果表明，Mus308对于氧和N-乙基化损伤的处理、取决于诱导DNA损伤水平的受精卵存活以及DNA损伤邻近序列的影响是必需的。这些结果支持了mus308在与跨损伤合成途径以及替代末端连接系统相关的耐受机制中的作用。
