Notch信号通路在人牙髓细胞衰老中的作用
Involvement of Notch signalling pathway in senescence of human dental pulp cells.
作者信息
Zou Xiao Ying, Zhuang Heng, Yue Lin, Gao Xue Jun
机构信息
Department of Cariology and Endodontology, Peking University School and Hospital of Stonmatology, Beijing, PR China.
出版信息
Chin J Dent Res. 2010;13(1):45-9.
OBJECTIVE
To evaluate whether the Notch signalling pathway is involved in the senescence of human dental pulp cells.
METHODS
Human dental pulp cells were isolated and cultured. The Notch signalling pathway was blocked by adding DAPT (N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester, γ-secretase inhibitor, 5 μmol/L) into the culture medium. Cell proliferation was evaluated by MTT assay. Cell senescence was evaluated through expression of senescenceassociated β-galactosidase (SA-β-Gal) using β-galactosidase staining.
RESULTS
The proliferation of the human dental pulp cells decreased dramatically after treatment with DAPT, compared with the control. SA-β-Gal positive staining cells were seen in the DAPT group, but not in the control group.
CONCLUSION
The Notch signalling pathway is involved in the senescence of human dental pulp cells.
目的
评估Notch信号通路是否参与人牙髓细胞的衰老过程。
方法
分离培养人牙髓细胞。通过向培养基中添加DAPT（N-[N-(3,5-二氟苯乙酰基-L-丙氨酰)]-S-苯基甘氨酸叔丁酯，γ-分泌酶抑制剂，5 μmol/L）来阻断Notch信号通路。采用MTT法评估细胞增殖。使用β-半乳糖苷酶染色通过衰老相关β-半乳糖苷酶（SA-β-Gal）的表达评估细胞衰老。
结果
与对照组相比，用DAPT处理后人牙髓细胞的增殖显著降低。在DAPT组中可见SA-β-Gal阳性染色细胞，而对照组中未见。
结论
Notch信号通路参与人牙髓细胞的衰老过程。
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