Deutsches Krebsforschungszentrum, Molecular Genetics of Breast Cancer, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.
Breast Cancer Res Treat. 2011 May;127(1):259-64. doi: 10.1007/s10549-010-1194-3. Epub 2010 Oct 9.
The human homolog of the Drosophila Scribble (SCRIB) tumor suppressor gene encodes a protein that regulates apical-basolateral polarity in mammalian epithelia and controls cell proliferation. Due to the role of cell polarity proteins in human cancers, we investigated whether genetic variability in SCRIB impacts breast carcinogenesis and tumor pathology. Five genetic variants were analyzed for an association with breast cancer risk and histopathological tumor parameters using a single nucleotide polymorphism (SNP) tagging approach. Genotyping of five tag SNPs was performed by TaqMan allelic discrimination and RFLP-based PCR using the GENICA population-based breast cancer case-control collection including 1,021 cases and 1,015 age-matched controls. Odds ratios (OR) and 95% confidence intervals (CI) were calculated by ordinal logistic regression. None of the tag SNPs was associated with breast cancer risk or tumor characteristics. Our findings suggest that genetic variability in the SCRIB polarity gene does not contribute to breast cancer development.
果蝇 Scribble（SCRIB）肿瘤抑制基因的人类同源物编码一种蛋白，该蛋白在哺乳动物上皮细胞中调节顶端-基底极性，并控制细胞增殖。由于细胞极性蛋白在人类癌症中的作用，我们研究了 SCRIB 中的遗传变异是否会影响乳腺癌的发生和肿瘤病理学。使用单核苷酸多态性（SNP）标记方法，分析了五个遗传变异与乳腺癌风险和组织病理学肿瘤参数的关联。通过 TaqMan 等位基因鉴别和基于 RFLP 的 PCR 对五个标记 SNP 进行基因分型，使用基于 GENICA 的基于人群的乳腺癌病例对照集进行，该集合包括 1021 例病例和 1015 例年龄匹配的对照。通过有序逻辑回归计算比值比（OR）和 95%置信区间（CI）。没有一个标记 SNP 与乳腺癌风险或肿瘤特征相关。我们的研究结果表明，SCRIB 极性基因中的遗传变异不会导致乳腺癌的发生。
