Department of Chemistry, Stanford University, Stanford, California 94305, USA.
Microsc Res Tech. 2011 Jun;74(6):496-501. doi: 10.1002/jemt.20937. Epub 2010 Oct 8.
We report a microfluidic positioning chamber (MPC) that can rapidly and repeatedly relocate the same imaging area on a microscope stage. The "roof" of the microfluidic chamber was printed with serials of coordinate numbers that act as positioning marks for mammalian cells that grow attached to the "floor" of the microfluidic chamber. MPC cell culture chamber provided a simple solution for tracking the same cell or groups of cells over days or weeks. The positioning marks were used to register time-lapse images of the same imaging area to single-pixel accuracy. Using MPC cell culture chamber, we tracked the migration, division, and differentiation of individual PC12 cells for over a week using bright field and fluorescence imaging.
我们报告了一种微流定位腔（MPC），它可以快速且反复地将显微镜载物台上的同一成像区域重新定位。微流腔的“顶”部印有一系列坐标数字，作为附着在微流腔“底”部的哺乳动物细胞的定位标记。MPC 细胞培养室为跟踪同一细胞或多组细胞数天或数周提供了一种简单的解决方案。这些定位标记用于将同一成像区域的延时图像注册到单像素精度。使用 MPC 细胞培养室，我们使用明场和荧光成像，跟踪单个 PC12 细胞的迁移、分裂和分化超过一周。
