Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
J Neurosci Res. 2010 Dec;88(16):3540-6. doi: 10.1002/jnr.22514. Epub 2010 Oct 8.
The role of matrix metalloproteinases (MMPs) and cytokines in the pathogenesis of Guillain-Barré syndrome (GBS) largely remains unknown. We studied the role of MMP-2, MMP-9, tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) in disease progression and recovery of patients with GBS. Sixty-five patients with GBS and 68 healthy controls were enrolled in the study. Serum levels of MMP-2, MMP-9, TNF-α, and IL-1β were analyzed by ELISA, and activities of MMPs were measured by zymography. Expression of MMP-9, TNF-α, and IL-1β was higher in the progressive phase and lower in the recovery phase of GBS than in controls. A positive correlation of MMP-2 with IL-1β and MMP-9 with TNF-α and IL-1β was observed with progressive-phase GBS. The study shows that up-regulation of MMP-9 along with proinflammatory cytokines (TNF-α and IL-1β) in the early course appears to be associated with immune-mediated disease progression resulting from inflammation in the peripheral nervous system, whereas, during the later phase, down-regulation of MMP-9 and proinflammatory cytokines is implicated in recovery from the disease.
基质金属蛋白酶（MMPs）和细胞因子在格林-巴利综合征（GBS）发病机制中的作用在很大程度上尚不清楚。我们研究了 MMP-2、MMP-9、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）在 GBS 患者疾病进展和恢复中的作用。研究纳入了 65 例 GBS 患者和 68 名健康对照者。通过 ELISA 分析血清 MMP-2、MMP-9、TNF-α和 IL-1β水平，并通过酶谱法测量 MMPs 的活性。与对照组相比，MMP-9 在 GBS 的进展期更高，在恢复期更低。在进展期 GBS 中观察到 MMP-2 与 IL-1β呈正相关，MMP-9 与 TNF-α和 IL-1β呈正相关。本研究表明，在早期，MMP-9 与促炎细胞因子（TNF-α和 IL-1β）的上调似乎与炎症引起的外周神经系统免疫介导的疾病进展有关，而在后期，MMP-9 和促炎细胞因子的下调与疾病的恢复有关。
