Xie Ke-Liang, Hou Li-Chao, Wang Guo-Lin, Xiong Li-Ze
Department of Anesthesiology, General Hospital of Tianjin Medical University, Tianjin 300052, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2010 Sep;39(5):454-7. doi: 10.3785/j.issn.1008-9292.2010.05.002.
To investigate the effect of hydrogen gas inhalation on survival rate and serum high mobility group box 1 (HMGB1) levels in severe septic mice.
Severe sepsis was induced by cecal ligation and puncture (CLP) operation in mice.A total of 248 mice were randomly divided into four groups: sham operation group (sham), sham operation with hydrogen gas inhalation group (sham+H2), severe CLP group (severe CLP) and severe CLP with hydrogen gas inhalation group (severe CLP+H2). Hydrogen gas inhalation was given for 1 h at 1st and 6th h after CLP or sham operation, respectively. The survival rates and serum HMGB1 levels of all groups at different time points were measured.
The 7-d survival rates of severe CLP mice was 0 % (Compared with Sham group, P <0.05), and the serum HMBG1 levels from h2 to h32 after CLP operation were significantly increased in severe CLP mice (Compared with Sham group, P <0.05). Hydrogen gas treatment increased the 7-d survival rate of severe CLP mice to 60 % (Compared with severe sepsis group, P <0.05) and significantly reduced the serum HMGB1 levels at different time points (Compared with severe sepsis group, P <0.05).
Hydrogen gas inhalation can decrease the serum HMGB1 levels and increase the survival rate of rats with severe sepsis.
探讨氢气吸入对严重脓毒症小鼠存活率及血清高迁移率族蛋白B1（HMGB1）水平的影响。
采用盲肠结扎穿孔（CLP）手术诱导小鼠发生严重脓毒症。将248只小鼠随机分为四组：假手术组（sham）、假手术氢气吸入组（sham+H2）、严重CLP组（severe CLP）和严重CLP氢气吸入组（severe CLP+H2）。分别在CLP或假手术后第1小时和第6小时给予氢气吸入1小时。测量所有组在不同时间点的存活率和血清HMGB1水平。
严重CLP小鼠的7天存活率为0%（与假手术组相比，P<0.05），CLP手术后2小时至32小时，严重CLP小鼠血清HMBG1水平显著升高（与假手术组相比，P<0.05）。氢气治疗使严重CLP小鼠的7天存活率提高到60%（与严重脓毒症组相比，P<0.05），并显著降低了不同时间点的血清HMGB1水平（与严重脓毒症组相比，P<0.05）。
氢气吸入可降低严重脓毒症大鼠血清HMGB1水平，提高其存活率。
