Primate Genetics Laboratory, German Primate Center, Göttingen, Germany.
Immunogenetics. 2011 Feb;63(2):85-93. doi: 10.1007/s00251-010-0487-3. Epub 2010 Oct 12.
We here report the genomic organisation of the grey mouse lemur (Microcebus murinus) MHC class II DQ and DR region based on BAC clone analysis. The sequenced Mimu-MHC haplotype spans 343 kb and encompasses the genes TAP2, DOB, DQB, DQA, DRB, DRA, BTNL2 and a further BTNL gene. The DQ and DR genes of this haplotype are not duplicated. Mimu-DOB is not transcribed and represents a pseudogene due to deletions and premature stop codons. Analysis of BAC clone DNA, a cDNA sample and eight genomic DNA samples suggests that Mimu-DRB, Mimu-DQA and Mimu-DQB are highly polymorphic with the majority of peptide-binding residues being affected by polymorphisms. In contrast, Mimu-DRA is moderately polymorphic, and the variable amino acid positions are not part of the peptide-binding region. Phylogenetic analysis of Mimu-DQA and Mimu-DQB and other primate DQA and DQB genes indicates that duplication of DQA and DQB loci occurred in Anthropoidea after the split from Strepsirrhini.
我们在此报道了基于 BAC 克隆分析的灰鼠狐猴（Microcebus murinus）MHC Ⅱ类 DQ 和 DR 区的基因组组织。测序的 Mimu-MHC 单体型跨越 343kb，包含 TAP2、DOB、DQB、DQA、DRB、DRA、BTNL2 和另一个 BTNL 基因。该单体型的 DQ 和 DR 基因没有重复。由于缺失和提前终止密码子，Mimu-DOB 不转录，代表一个假基因。对 BAC 克隆 DNA、cDNA 样本和 8 个基因组 DNA 样本的分析表明，Mimu-DRB、Mimu-DQA 和 Mimu-DQB 高度多态性，大多数肽结合残基受多态性影响。相比之下，Mimu-DRA 中度多态性，可变氨基酸位置不是肽结合区的一部分。Mimu-DQA 和 Mimu-DQB 与其他灵长类动物 DQA 和 DQB 基因的系统发育分析表明，在从树鼩目中分离出来后，DQA 和 DQB 基因座发生了复制。
