Cui X, Zhang J, Zhou Y, Hu S, Zhang J, Gao M, Zhang J
Institute of Gerontology , General Hospital of Chinese PLA , Beijing 100853 , P. R. China.
Zhongguo Fei Ai Za Zhi. 2000 Apr 20;3(2):104-6. doi: 10.3779/j.issn.1009-3419.2000.02.08.
To investigate the role of telomerase activity in the oncogenesis and progression of human lung cancer.
A total of 50 malignant neoplasm's specimens frompatient with lung cancer including 36 surgically resected tissues , 7 fiberobronchoscopically collected tissues or cells , 7 pleural effusion cells and 20 tumor-adjacent tissues and 5 tuberculous tissues or cells were examined for telomerase activity by PCR-based TRAP(telomeric repeat amplification protocol) assay.
In the lung cancer group , the positive rate of telomerase activity in surgically resected lung cancer tissues was 91. 7 %(33 out of 36 samples) , and 85. 7 % (6 out of 7 samples) and 71. 4 % (5 out of 7 samples) in fiberobronchoscopically collected tissues or cells and pleural effusion cells , respectively. However , that in the tumor-adjacent tissues was 10 % (2 out of 20 specimens) and no telomerase activity was observed in 5 tuberculous tissues or cells. Positive rate in telomerase activity (85. 7 %) was significantly higher than that in pathologic assay (42. 9 %) in the same 7 samples collected through fiberobronchoscope.
The results suggest that detection of telomerase activity of fiberobronchoscopically collected tissues or cells and pleural effusion cells would be a helpful examination in the diagnosis of patients with lung cancer.
探讨端粒酶活性在人类肺癌发生发展中的作用。
采用基于聚合酶链反应的端粒重复序列扩增法（TRAP）检测50例肺癌患者的恶性肿瘤标本，其中包括36例手术切除组织、7例经纤维支气管镜采集的组织或细胞、7例胸腔积液细胞、20例癌旁组织以及5例结核组织或细胞的端粒酶活性。
肺癌组中，手术切除的肺癌组织中端粒酶活性阳性率为91.7%（36例样本中有33例），经纤维支气管镜采集的组织或细胞中端粒酶活性阳性率为85.7%（7例样本中有6例），胸腔积液细胞中端粒酶活性阳性率为71.4%（7例样本中有5例）。然而，癌旁组织中端粒酶活性阳性率为10%（20例标本中有2例），5例结核组织或细胞未检测到端粒酶活性。在通过纤维支气管镜采集的相同7例样本中，端粒酶活性阳性率（85.7%）显著高于病理检测阳性率（42.9%）。
结果表明，检测经纤维支气管镜采集的组织或细胞以及胸腔积液细胞的端粒酶活性对肺癌患者的诊断有帮助。
