由轴上单色仪调谐的用于荧光寿命成像的超连续谱源。

Supercontinuum source tuned by an on-axis monochromator for fluorescence lifetime imaging.

作者信息

Mercatelli Raffaella, Soria Silvia, Molesini Giuseppe, Bianco Federica, Righini Giancarlo, Quercioli Franco

机构信息

ISC-CNR, Istituto dei Sistemi Complessi, Sesto Fiorentino (FI), Italy.

出版信息

Opt Express. 2010 Sep 13;18(19):20505-11. doi: 10.1364/OE.18.020505.

DOI:10.1364/OE.18.020505

PMID:20940943

Abstract

We report on the application of an optically tunable coherent white light source, based on the supercontinuum generation from microstructured optical fibres, to fluorescence lifetime imaging (FLIM) microscopy and Foerster resonance energy transfer (FRET). A prototype lens based on monotonic longitudinal chromatic aberration is used for tuning the supercontinuum wavelengths from 400 to 1000 nm and acts as an axial monochromator, suitable for fibre delivery in confocal microscopy.

摘要

我们报告了一种基于微结构光纤产生超连续谱的光学可调谐相干白光源在荧光寿命成像（FLIM）显微镜和福斯特共振能量转移（FRET）中的应用。一种基于单调纵向色差的原型透镜用于将超连续谱波长从400纳米调谐到1000纳米，并用作轴向单色仪，适用于共聚焦显微镜中的光纤传输。