丹参对重症急性胰腺炎或阻塞性黄疸大鼠肠黏膜细胞凋亡及 NF-κB 蛋白表达的影响。

Effect of Danshen on apoptosis and NF-κB protein expression of the intestinal mucosa of rats with severe acute pancreatitis or obstructive jaundice.

机构信息

Department of General Surgery, Hangzhou First People's Hospital, Hangzhou 310006, China.

出版信息

Hepatobiliary Pancreat Dis Int. 2010 Oct;9(5):537-46.

PMID:20943465

Abstract

BACKGROUND

Intestinal mucosa injury in cases of severe acute pancreatitis (SAP) or obstructive jaundice (OJ) is one of the main reasons for the accelerated aggravation of these diseases. Besides being an organ to digest and absorb nutrients, the intestine is also a unique immune organ. When SAP and OJ develop, the destruction of the intestinal mucosa barrier is an important contributing factor for the development of bacterial translocation, systemic inflammatory response syndrome, and multiple organ dysfunction syndrome. It is important to protect the intestinal mucosa in the therapy for SAP and OJ. In this study, we determined the effect of Radix Salviae Miltiorrhizae (Danshen) injection on apoptosis and NF-κB P65 protein expression in the intestinal mucosa of rats with SAP or OJ, and explored the protective mechanism of Danshen in their mucosa.

METHODS

Sprague-Dawley rats were used in the SAP and OJ experiments. These rats were randomly divided into sham-operated, model control, and treated groups. At various times after operation, the mortality rates were calculated. Subsequently, the rats were killed to assess the pathological changes, the expression levels of Bax and NF-κB proteins, and the apoptosis indices in the intestinal mucosa.

RESULTS

Compared to the corresponding model control group, the number of SAP or OJ rats that died in the treated group decreased but showed no statistically significant difference. At all time points after operation, there was no significant difference between the treated and model control groups in the staining intensity as well as the product of staining intensity and positive staining rate of Bax protein in the intestinal mucosa of SAP and OJ rats . At 3 hours after operation, the apoptosis index of the intestinal mucosa of SAP rats in the treated group was lower than that in the model control group (P<0.01). At 12 hours after operation in SAP rats and 28 days after operation in OJ rats, the staining intensity as well as the product of staining intensity and positive staining rate of NF-κB protein of the intestinal mucosa in the treated group were lower than those in the model control group (P<0.01).

CONCLUSION

Danshen exerts protective effects on the intestinal mucosa of SAP and OJ rats perhaps by inhibiting apoptosis and down-regulating NF-κB protein.

摘要

背景

严重急性胰腺炎（SAP）或梗阻性黄疸（OJ）患者的肠道黏膜损伤是这些疾病加速恶化的主要原因之一。肠道不仅是消化吸收营养物质的器官，也是独特的免疫器官。SAP 和 OJ 发展时，肠黏膜屏障的破坏是细菌易位、全身炎症反应综合征和多器官功能障碍综合征发展的重要因素。保护 SAP 和 OJ 治疗中的肠黏膜非常重要。在这项研究中，我们确定了丹参注射液对 SAP 或 OJ 大鼠肠黏膜细胞凋亡和 NF-κB P65 蛋白表达的影响，并探讨了丹参在其黏膜中的保护机制。

方法

采用 SAP 和 OJ 实验的 Sprague-Dawley 大鼠。这些大鼠随机分为假手术组、模型对照组和治疗组。术后不同时间计算死亡率。然后处死大鼠，评估肠道黏膜的病理变化、Bax 和 NF-κB 蛋白的表达水平以及肠黏膜的凋亡指数。

结果

与相应的模型对照组相比，治疗组 SAP 或 OJ 大鼠的死亡人数减少，但无统计学差异。术后所有时间点，SAP 和 OJ 大鼠肠黏膜 Bax 蛋白染色强度及其染色强度与阳性染色率乘积在治疗组与模型对照组之间无显著差异。术后 3 小时，SAP 大鼠肠黏膜的凋亡指数低于模型对照组（P<0.01）。SAP 大鼠术后 12 小时和 OJ 大鼠术后 28 天，治疗组肠黏膜 NF-κB 蛋白的染色强度及其染色强度与阳性染色率乘积均低于模型对照组（P<0.01）。