人细小病毒 B19 衣壳基因的密码子优化极大地提高了其在非允许细胞中的表达。
Codon optimization of human parvovirus B19 capsid genes greatly increases their expression in nonpermissive cells.
机构信息
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
出版信息
J Virol. 2010 Dec;84(24):13059-62. doi: 10.1128/JVI.00912-10. Epub 2010 Oct 13.
Abstract
Parvovirus B19 (B19V) is pathogenic for humans and has an extreme tropism for human erythroid progenitors. We report cell type-specific expression of the B19V capsid genes (VP1 and VP2) and greatly increased B19V capsid protein production in nonpermissive cells by codon optimization. Codon usage limitation, rather than promoter type and the 3' untranslated region of the capsid genes, appears to be a key factor in capsid protein production in nonpermissive cells. Moreover, B19 virus-like particles were successfully generated in nonpermissive cells by transient transfection of a plasmid carrying both codon-optimized VP1 and VP2 genes.
摘要
细小病毒 B19（B19V）对人类具有致病性，并且对人类红系祖细胞具有极强的嗜性。我们报告了 B19V 衣壳基因（VP1 和 VP2）在细胞类型特异性表达，并通过密码子优化极大地增加了非许可细胞中的 B19V 衣壳蛋白产生。密码子使用限制，而不是启动子类型和衣壳基因的 3'非翻译区，似乎是在非许可细胞中产生衣壳蛋白的关键因素。此外，通过瞬时转染携带密码子优化的 VP1 和 VP2 基因的质粒，在非许可细胞中成功生成了 B19 病毒样颗粒。
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本文引用的文献
1
Fine-tuning translation kinetics selection as the driving force of codon usage bias in the hepatitis A virus capsid.
PLoS Pathog. 2010 Mar 5;6(3):e1000797. doi: 10.1371/journal.ppat.1000797.
2
Ex vivo-generated CD36+ erythroid progenitors are highly permissive to human parvovirus B19 replication.
J Virol. 2008 Mar;82(5):2470-6. doi: 10.1128/JVI.02247-07. Epub 2007 Dec 26.
3
Molecular and functional analyses of a human parvovirus B19 infectious clone demonstrates essential roles for NS1, VP1, and the 11-kilodalton protein in virus replication and infectivity.
J Virol. 2006 Jun;80(12):5941-50. doi: 10.1128/JVI.02430-05.
4
Plasmid encoding papillomavirus Type 16 (HPV16) DNA constructed with codon optimization improved the immunogenicity against HPV infection.
Vaccine. 2004 Dec 16;23(5):629-38. doi: 10.1016/j.vaccine.2004.07.010.
5
Codon optimization and mRNA amplification effectively enhances the immunogenicity of the hepatitis C virus nonstructural 3/4A gene.
Gene Ther. 2004 Mar;11(6):522-33. doi: 10.1038/sj.gt.3302184.
6
Effects of codon-optimization on protein expression by the human herpesvirus 6 and 7 U51 open reading frame.
J Virol Methods. 2003 Aug;111(2):145-56. doi: 10.1016/s0166-0934(03)00173-3.
7
Human parvovirus B19 induces cell cycle arrest at G(2) phase with accumulation of mitotic cyclins.
J Virol. 2001 Aug;75(16):7555-63. doi: 10.1128/JVI.75.16.7555-7563.2001.
8
Infection of the erythroid cell line, KU812Ep6 with human parvovirus B19 and its application to titration of B19 infectivity.
J Virol Methods. 1999 Dec;83(1-2):45-54. doi: 10.1016/s0166-0934(99)00105-6.
9
The 3' untranslated region of the B19 parvovirus capsid protein mRNAs inhibits its own mRNA translation in nonpermissive cells.
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10
Codon frequencies in 119 individual genes confirm consistent choices of degenerate bases according to genome type.
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