Yan Jiang Xi Lu, No 107, Department of Pediatrics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, PR China.
Anticancer Res. 2010 Sep;30(9):3493-9.
The efficacy of various combinations of stem cell factor (SCF), FLT3 ligand, interleukin (IL)-2, IL-7 and IL-15 to induce and expand cord blood-derived cytokine-induced killer (CIK) cells was investigated. There were three treatment groups: group A: SCF combined with IL-2, IL-7 and IL-15; group B: SCF, FLT3 ligand combined with IL-2, IL-7 and IL-15, and group C: IL-2, IL-7 and IL-15, the control group. Proliferation rates of CD3(+)CD56(+) CIK cells and CD3(-)CD56(+) natural killer (NK) cells were highest in group B; expansion of CIK cells increased 796.1 ± 278.5-fold, and that of NK cells increased 36.6 ± 3.5-fold. All expanded cord blood-derived CIK/NK cells showed cytotoxic activity against the K562 cell line. Interestingly, the cytotoxicity of group A was highest and significantly higher than that of other groups. These protocols might provide an alternative choice for CIK/NK cell expansion.
研究了干细胞因子 (SCF)、FLT3 配体、白细胞介素 (IL)-2、IL-7 和 IL-15 各种组合对诱导和扩增脐血来源的细胞因子诱导的杀伤 (CIK) 细胞的疗效。有三组治疗组：A 组：SCF 联合 IL-2、IL-7 和 IL-15；B 组：SCF、FLT3 配体联合 IL-2、IL-7 和 IL-15，C 组：IL-2、IL-7 和 IL-15，对照组。B 组 CD3(+)CD56(+)CIK 细胞和 CD3(-)CD56(+)自然杀伤 (NK) 细胞的增殖率最高；CIK 细胞扩增 796.1 ± 278.5 倍，NK 细胞扩增 36.6 ± 3.5 倍。所有扩增的脐血来源的 CIK/NK 细胞对 K562 细胞系均表现出细胞毒性。有趣的是，A 组的细胞毒性最高，明显高于其他组。这些方案可能为 CIK/NK 细胞扩增提供另一种选择。
