Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA.
Analyst. 2010 Dec;135(12):3030-3. doi: 10.1039/c0an00546k. Epub 2010 Oct 13.
Confocal Raman micro-spectral imaging (CRMI) is a relatively novel technique for the construction of label-free images of biological entities, such as cells or tissue sections. This method utilizes thousands of spatially resolved Raman spectra, and sophisticated image analysis algorithms, to construct images which are based strictly on the inherent biochemical abundance contrast afforded by Raman microscopy. Here, we apply this methodology to monitor the very early communication processes that occur in a growing stem cell colony, yielding information on biochemical composition during development processes of the early stages of embryogenesis.
共焦拉曼微光谱成像（CRMI）是一种相对较新的技术，可用于构建生物实体（如细胞或组织切片）的无标记图像。该方法利用数千个空间分辨拉曼光谱和复杂的图像分析算法，构建严格基于拉曼显微镜提供的固有生化丰度对比的图像。在这里，我们应用这种方法来监测在不断生长的干细胞菌落中发生的早期通讯过程，从而提供胚胎发生早期发育过程中生化组成的信息。
