McClure T D
Novartis Agricultural Discovery Institute, Inc., San Diego, California, USA.
Curr Protoc Toxicol. 2001 May;Chapter 4:Unit4.4. doi: 10.1002/0471140856.tx0404s03.
This unit provides a discussion of high-pressure liquid chromatography-atmospheric pressure ionization mass spectrometry (LC-API-MS) for separation, detection, and identification of products from xenobiotic metabolism. It includes an introduction and important technical information for electrospray and atmospheric pressure chemical ionization methods as well as scan modes. Emphasis has been placed on each method as related to phase I and phase II metabolite identification and there are listings of specific applications (and references) to reduce method development time.
本单元讨论了用于分离、检测和鉴定外源化合物代谢产物的高压液相色谱 - 大气压电离质谱法（LC - API - MS）。它包括电喷雾和大气压化学电离方法以及扫描模式的介绍和重要技术信息。重点介绍了与I相和II相代谢物鉴定相关的每种方法，并列出了具体应用（及参考文献），以减少方法开发时间。
