Measurement of aryl and alcohol sulfotransferase activity.

Two methods are presented for determining the catalytic activity of aryl and alcohol sulfotransferases. The first assay is a simple and rapid procedure that is based on the extraction of a paired ion formed between the product organic sulfate and methylene blue. The second method employs HPLC analysis of the substrate-dependent formation of PAP, an assay that is particularly useful when the sulfuric acid ester product is chemically unstable.