芳基和醇磺基转移酶活性的测定。
Measurement of aryl and alcohol sulfotransferase activity.
作者信息
Sheng J J, Sharma V, Duffel M W
机构信息
University of Iowa, Iowa City, Iowa, USA.
出版信息
Curr Protoc Toxicol. 2001 Aug;Chapter 4:Unit4.5. doi: 10.1002/0471140856.tx0405s08.
PMID:20945296
Abstract
Two methods are presented for determining the catalytic activity of aryl and alcohol sulfotransferases. The first assay is a simple and rapid procedure that is based on the extraction of a paired ion formed between the product organic sulfate and methylene blue. The second method employs HPLC analysis of the substrate-dependent formation of PAP, an assay that is particularly useful when the sulfuric acid ester product is chemically unstable.
摘要
本文介绍了两种测定芳基和醇类磺基转移酶催化活性的方法。第一种测定方法是一种简单快速的程序，它基于对产物有机硫酸盐与亚甲蓝之间形成的配对离子的萃取。第二种方法采用高效液相色谱法分析依赖底物形成的对氨基苯磷酸（PAP），当硫酸酯产物化学性质不稳定时，该测定方法特别有用。
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