Kelley Michael, Vessey Donald A
Veterans Administration Medical Center, San Francisco, California, USA.
Curr Protoc Toxicol. 2003 Feb;Chapter 4:Unit4.11. doi: 10.1002/0471140856.tx0411s14.
A wide variety of xenobiotic carboxylic acids are metabolized to their amino acid conjugates via a pathway that exists primarily in liver and kidney. This conjugation occurs in a two-step pathway catalyzed by two distinct types of enzymes, ligases and transferases. Measurements of acyl-CoA ligase activity include monitoring the rate of appearance of AMP or PPi, or the CoA adduct. N-acyltransferases catalyze formation of an amino acid conjugate from the CoA-activated intermediate, releasing CoA. This reaction is monitored by following the release of free CoA or the disappearance of the acyl-CoA adduct.
