Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Macromol Biosci. 2011 Feb 11;11(2):211-8. doi: 10.1002/mabi.201000302. Epub 2010 Oct 13.
A series of amphiphilic multi-armed PPn copolymers were prepared by ROP of Phe-NCA with PEI-25k as a macroinitiator. The particle size of the PPn/DNA complexes was about 100 nm and the zeta potentials were below 20 mV. An MTT assay demonstrated that all the PPn copolymers had lower cytotoxicity compared to PEI-25k. In vitro gene transfection studies were also conducted in HeLa, 293 and CT 26 cells. The optimal quantity of hydrophobic phenylalanine segments in PP80 led to higher transfection efficiency in various cell lines based on this study. The results indicate that PP80 was the best candidate for gene delivery among these PPn copolymers.
一系列两亲性多臂 PPn 共聚物是通过 ROP 用 PEI-25k 作为大分子引发剂合成的。PPn/DNA 复合物的粒径约为 100nm，zeta 电位低于 20mV。MTT 试验表明，与 PEI-25k 相比，所有的 PPn 共聚物的细胞毒性都较低。还在 HeLa、293 和 CT26 细胞中进行了体外基因转染研究。本研究表明，在 PP80 中，疏水性苯丙氨酸片段的最佳数量导致在各种细胞系中具有更高的转染效率。结果表明，在这些 PPn 共聚物中，PP80 是基因传递的最佳候选物。
