Department of Physiology, Toho University School of Medicine, Tokyo, Japan.
J Cell Physiol. 2011 Jun;226(6):1433-8. doi: 10.1002/jcp.22436.
The isolation of neural stem cells (NSCs) has been hampered by the lack of valid cell-surface antigens on NSCs, and novel valuable markers have been proposed. Glycan (oligosaccharide chain) is a potential candidate as a marker to isolate NSCs, because the species and the combination order of saccharides in glycan generate remarkable structural diversity and specificity. At present, the expression of hundreds of glycoconjugates with glycans have been found in the NSCs; however, just a few glycan-epitopes have been identified as valuable cell-surface markers. This review focused on the isolation of NSC using glycoprotein, especially complex type N-glycans. The cell-surface N-glycan-mediated isolation of NSCs is therefore expected to provide a comprehensive understanding of the biologic characteristics of NSCs in the brain, and thereby help to develop novel strategies in the field of regenerative medicine.
神经干细胞（NSCs）的分离一直受到 NSCs 表面缺乏有效抗原的阻碍，因此提出了新的有价值的标记物。糖链（寡糖链）作为一种分离 NSCs 的标记物具有潜在的应用价值，因为糖链中糖的种类和组合顺序产生了显著的结构多样性和特异性。目前，已经在 NSCs 中发现了数百种带有聚糖的糖缀合物的表达，但只有少数糖表位被鉴定为有价值的细胞表面标记物。本综述重点介绍了使用糖蛋白，特别是复杂型 N-糖链来分离 NSC。因此，细胞表面 N-糖链介导的 NSC 分离有望为理解大脑中 NSC 的生物学特性提供全面的认识，从而有助于在再生医学领域开发新的策略。
