Hadfield Catherine A, Haines Ashley N, Clayton Leigh A, Whitaker Brent R
National Aquarium, 501 East Pratt Street, Baltimore, Maryland 21202, USA.
J Zoo Wildl Med. 2010 Sep;41(3):480-6. doi: 10.1638/2009-0236.1.
The transfusion of whole blood in elasmobranchs could provide cardiovascular support following hemorrhage. Since donor and recipient compatibility is not known, a technique was established to allow cross matching of red blood cells and serum in sharks. Cross matching was carried out among 19 individuals from seven species: the nurse shark (Ginglymostoma cirratum), sandbar shark (Carcharhinus plumbeus), sandtiger shark (Carcharias taurus), white-spotted bamboo shark (Chiloscyllium plagiosum), brown-banded bamboo shark (Chiloscyllium punctatum), zebra shark (Stegostoma fasciatum), and spotted wobbegong (Orectolobus maculatus). Negative cross-matches showed no agglutination or hemolysis, suggesting that donor and recipient would be compatible. Cross-matches between conspecifics were all negative (sandbar, sandtiger, nurse, and white-spotted bamboo sharks). All cross-matches between sandbar and sandtiger sharks were also negative. Positive crossmatches consisted of agglutination or hemolysis of red blood cells, suggesting that the donor and recipient would be incompatible. Strong positive reactions occurred, for example, with red blood cells from sandtiger and sandbar sharks and serum from nurse sharks. Cross matching should be carried out in elasmobranchs prior to any blood transfusion.
在板鳃亚类动物中，全血输血可在出血后提供心血管支持。由于供体和受体的相容性未知，因此建立了一种技术来实现鲨鱼红细胞与血清的交叉配血。交叉配血在来自七个物种的19个个体中进行：护士鲨（Ginglymostoma cirratum）、沙洲鲨（Carcharhinus plumbeus）、沙虎鲨（Carcharias taurus）、白点竹鲨（Chiloscyllium plagiosum）、褐带竹鲨（Chiloscyllium punctatum）、斑马鲨（Stegostoma fasciatum）和斑点须鲨（Orectolobus maculatus）。阴性交叉配血未出现凝集或溶血现象，表明供体和受体是相容的。同种个体之间的交叉配血均为阴性（沙洲鲨、沙虎鲨、护士鲨和白点竹鲨）。沙洲鲨和沙虎鲨之间的所有交叉配血也均为阴性。阳性交叉配血表现为红细胞凝集或溶血，表明供体和受体不相容。例如，沙虎鲨和沙洲鲨的红细胞与护士鲨的血清之间会出现强烈的阳性反应。在对板鳃亚类动物进行任何输血之前，都应进行交叉配血。
