Roh Yoon-Seok, Park Heejin, Cho Ara, Islam Mohammad Rafiqul, Chekarova Irina, Ejaz Sohail E, Lim Chae Woong, Kim Bumseok
Bio-safety Research Institute and College of Veterinary Medicine, Chonbuk National University, Jeonju City, 561-756, South Korea.
J Zoo Wildl Med. 2010 Sep;41(3):550-4. doi: 10.1638/2009-0237.1.
A 25-yr-old male freshwater crocodile (Crocodylus johnstoni) was diagnosed with pulmonary mycobacteriosis caused by Mycobacterium szulgai. Necropsy revealed fibrinous exudate in the right pleural cavity and white miliary nodules in the right lung lobe. Histopathologic examination revealed well-demarcated granulomas consisting of multinucleated giant cells and epithelioid cells surrounded by fibrous connective tissue. Atypically, lymphocytes had accumulated in the outer region of fibrous connective tissue. Mycobacterial infection was confirmed by nested polymerase chain reaction targeting the hsp65 gene and by Fite's method for detection of acid-fast bacilli within formalin-fixed, paraffin-embedded lung tissue. Sequence analysis of the DNA amplicon revealed that the species of mycobacterium shared 98% homology with the gene encoding the hsp65 gene of M. szulgai. This is the first report of M. szulgai as the causative agent of mycobacteriosis in a reptile.
一只25岁的雄性淡水鳄（约翰斯顿鳄）被诊断出患有由苏尔加伊分枝杆菌引起的肺部分枝杆菌病。尸检发现右胸腔有纤维蛋白渗出物，右肺叶有白色粟粒状结节。组织病理学检查显示，肉芽肿界限清晰，由多核巨细胞和上皮样细胞组成，周围是纤维结缔组织。不同寻常的是，淋巴细胞聚集在了纤维结缔组织的外部区域。通过针对hsp65基因的巢式聚合酶链反应以及福尔马林固定、石蜡包埋的肺组织中检测抗酸杆菌的菲茨方法，证实了分枝杆菌感染。对DNA扩增子的序列分析表明，该分枝杆菌物种与苏尔加伊分枝杆菌hsp65基因编码基因具有98%的同源性。这是苏尔加伊分枝杆菌作为爬行动物分枝杆菌病病原体的首次报道。
