Laboratory of Stem Cell and Developmental Biology, CHA Stem Cell Institute, CHA University, Seoul, Republic of Korea.
Stem Cells Dev. 2011 Jun;20(6):1063-9. doi: 10.1089/scd.2010.0385. Epub 2010 Nov 30.
The restricted gene expression of a differentiated cell can be reversed by forming hybrid with embryonic stem cells (ESCs). The resulting hybrid cells showed not only an ESC-specific marker expression but also a differentiation potential similar to the pluripotent fusion partner. Here, we evaluated whether the tetraploid fusion hybrid cells have a unique differentiation potential compared with diploid pluripotent cells. The first Oct4-GFP-positive cells were observed at day 2 following fusion between ESCs and neurosphere cells (OG2(+/-)/ROSA26(+/-)). Reprogramming efficiency was as high as 94.5% at passage 5 and 96.4% at passage 13. We have found that the tetraploid hybrid cells could form chimera with contribution to placenta after blastocyst injection. This result indicates that the tetraploid pluripotent fusion hybrid cells have wide range of differentiation potential. Therefore, we suggest that once the somatic cells are reprogrammed by fusion with ESCs, the tetraploid hybrid cells contributed to the extraembryonic as well as embryonic tissues.
已分化细胞的基因表达受到限制，可通过与胚胎干细胞（ESCs）形成杂交来逆转。由此产生的杂交细胞不仅表现出 ESC 特异性标志物的表达，而且还具有类似于多能融合伙伴的分化潜能。在这里，我们评估了四倍体融合杂交细胞与二倍体多能细胞相比是否具有独特的分化潜能。在 ESC 和神经球细胞（OG2(+/-)/ROSA26(+/-)）融合后第 2 天观察到第一个 Oct4-GFP 阳性细胞。在第 5 代和第 13 代时，重编程效率高达 94.5%和 96.4%。我们发现，四倍体杂交细胞在胚泡注射后可以形成胎盘嵌合体。这一结果表明，四倍体多能融合杂交细胞具有广泛的分化潜能。因此，我们认为一旦体细胞通过与 ESCs 融合被重编程，四倍体杂交细胞就会参与胚胎外和胚胎组织的形成。
