Chuang C Z, Ragan F A, Prasad C
Laboratory of Neurosciences, Pennington Biomedical Research Center, Baton Rouge, LA 70808-4124.
J Chromatogr. 1990 Dec 14;534:13-21. doi: 10.1016/s0378-4347(00)82144-1.
A reversed-phase high-performance liquid chromatographic (RP-HPLC) method for the separation of tryptophan and ten metabolites of tryptophan pyrrolase pathway has been developed by sequential optimization of mobile phase, by adjusting the pH, the concentration of triethylamine and the gradient elution. The baseline resolution of the compounds, by this optimized procedure, is obtained with an analysis time, including the re-equilibration period, of less than 30 min. We believe this is the first RP-HPLC method that can separate tryptophan and ten of its metabolites in a single chromatographic run.
通过对流动相进行连续优化，调节pH值、三乙胺浓度和梯度洗脱，开发了一种反相高效液相色谱（RP-HPLC）方法，用于分离色氨酸及其色氨酸吡咯酶途径的十种代谢物。通过这种优化程序，这些化合物在不到30分钟的分析时间内（包括重新平衡期）实现了基线分离。我们认为这是第一种能够在一次色谱运行中分离色氨酸及其十种代谢物的RP-HPLC方法。
