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Cryo-EM analysis reveals new insights into the mechanism of action of pyruvate carboxylase.
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A symmetrical tetramer for S. aureus pyruvate carboxylase in complex with coenzyme A.
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The role of biotin and oxamate in the carboxyltransferase reaction of pyruvate carboxylase.
Arch Biochem Biophys. 2014 Nov 15;562:70-9. doi: 10.1016/j.abb.2014.08.008. Epub 2014 Aug 23.
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Insights into the carboxyltransferase reaction of pyruvate carboxylase from the structures of bound product and intermediate analogs.
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Novel insights into the biotin carboxylase domain reactions of pyruvate carboxylase from Rhizobium etli.
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Allosteric Site at the Biotin Carboxylase Dimer Interface Mediates Activation and Inhibition in Pyruvate Carboxylase.
Biochemistry. 2023 Sep 5;62(17):2632-2644. doi: 10.1021/acs.biochem.3c00280. Epub 2023 Aug 21.
2
CryoEM structural exploration of catalytically active enzyme pyruvate carboxylase.
Nat Commun. 2022 Oct 19;13(1):6185. doi: 10.1038/s41467-022-33987-2.
3
Allosteric regulation alters carrier domain translocation in pyruvate carboxylase.
Nat Commun. 2018 Apr 11;9(1):1384. doi: 10.1038/s41467-018-03814-8.
4
Structural and functional studies of pyruvate carboxylase regulation by cyclic di-AMP in lactic acid bacteria.
Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):E7226-E7235. doi: 10.1073/pnas.1704756114. Epub 2017 Aug 14.
5
Functional conformations for pyruvate carboxylase during catalysis explored by cryoelectron microscopy.
Structure. 2014 Jun 10;22(6):911-22. doi: 10.1016/j.str.2014.04.011. Epub 2014 May 29.
6
A substrate-induced biotin binding pocket in the carboxyltransferase domain of pyruvate carboxylase.
J Biol Chem. 2013 Jul 5;288(27):19915-25. doi: 10.1074/jbc.M113.477828. Epub 2013 May 22.
7
Roles of Arg427 and Arg472 in the binding and allosteric effects of acetyl CoA in pyruvate carboxylase.
Biochemistry. 2012 Oct 16;51(41):8208-17. doi: 10.1021/bi301060d. Epub 2012 Oct 2.
8
Structure and function of biotin-dependent carboxylases.
Cell Mol Life Sci. 2013 Mar;70(5):863-91. doi: 10.1007/s00018-012-1096-0. Epub 2012 Aug 7.
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Regulation of the structure and activity of pyruvate carboxylase by acetyl CoA.
Arch Biochem Biophys. 2012 Mar 15;519(2):118-30. doi: 10.1016/j.abb.2011.11.015. Epub 2011 Nov 19.

本文引用的文献

1
A symmetrical tetramer for S. aureus pyruvate carboxylase in complex with coenzyme A.
Structure. 2009 Jun 10;17(6):823-32. doi: 10.1016/j.str.2009.04.008.
2
Decreased levels of metabolic enzymes in pancreatic islets of patients with type 2 diabetes.
Diabetologia. 2009 Jun;52(6):1087-91. doi: 10.1007/s00125-009-1319-6. Epub 2009 Mar 19.
3
Crystal structure of biotin carboxylase in complex with substrates and implications for its catalytic mechanism.
J Biol Chem. 2009 Apr 24;284(17):11690-7. doi: 10.1074/jbc.M805783200. Epub 2009 Feb 12.
4
The role of pyruvate carboxylase in insulin secretion and proliferation in rat pancreatic beta cells.
Diabetologia. 2008 Nov;51(11):2022-30. doi: 10.1007/s00125-008-1130-9. Epub 2008 Sep 4.
5
Structural evidence for substrate-induced synergism and half-sites reactivity in biotin carboxylase.
Protein Sci. 2008 Oct;17(10):1706-18. doi: 10.1110/ps.035584.108. Epub 2008 Aug 25.
7
Structure, mechanism and regulation of pyruvate carboxylase.
Biochem J. 2008 Aug 1;413(3):369-87. doi: 10.1042/BJ20080709.
8
Image processing for electron microscopy single-particle analysis using XMIPP.
Nat Protoc. 2008;3(6):977-90. doi: 10.1038/nprot.2008.62.
10
Protein structure fitting and refinement guided by cryo-EM density.
Structure. 2008 Feb;16(2):295-307. doi: 10.1016/j.str.2007.11.016.

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