National Center for Nanoscience and Technology, Laboratory of Nanobiomedicine and Nanosafety, Division of Nanomedicine and Nanobiology, No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China.
J Colloid Interface Sci. 2011 Jan 1;353(1):107-15. doi: 10.1016/j.jcis.2010.09.053. Epub 2010 Sep 25.
Novel amphiphilic copolymers based on poly(ε-caprolactone) (PCL) and hyperbranched poly (amine-ester) (HPAE) with various compositions were synthesized. The amphiphilic copolymers can self-assemble into nanoscopic micelles and their hydrophobic cores can encapsulate doxorubicin (DOX) in aqueous solutions. The DOX-loaded HPAE-co-PCL nanoparticles diameter increased from 121 to 184 nm with the increasing PCL segment in the copolymer composition. An in vitro study at 37°C demonstrated that DOX-release from nanoparticles at pH 5.0 was much faster than that at pH 7.4. The cytotoxicity for HeLa cells study demonstrated that DOX-loaded HPAE-co-PCL nanoparticles exhibited the anti-tumor effect was enhanced significantly, suggesting that the DOX-loaded HPAE-co-PCL nanoparticles have great potential as a tumor drug carrier.
新型两亲性共聚物基于聚（ε-己内酯）（PCL）和超支化聚（胺酯）（HPAE），具有不同的组成。两亲性共聚物可以自组装成纳米胶束，其疏水性核心可以在水溶液中包裹阿霉素（DOX）。载 DOX 的 HPAE-co-PCL 纳米粒子的直径从 121nm 增加到 184nm，随着共聚物组成中 PCL 段的增加。在 37°C 的体外研究表明，在 pH 5.0 时，纳米粒子中 DOX 的释放速度比在 pH 7.4 时快得多。对 HeLa 细胞的细胞毒性研究表明，载 DOX 的 HPAE-co-PCL 纳米粒子表现出显著增强的抗肿瘤作用，表明载 DOX 的 HPAE-co-PCL 纳米粒子具有作为肿瘤药物载体的巨大潜力。
