Department of Surgery, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa.
Pathol Res Pract. 2010 Dec 15;206(12):805-9. doi: 10.1016/j.prp.2010.08.004. Epub 2010 Oct 13.
Cholangiocarcinoma (CC) is a highly malignant epithelial cancer of the biliary tract, the cellular and molecular pathogenesis of which remains unclear. Malignant transformation of glandular epithelial cells is associated with the altered expression of mucin. We investigated the type of mucins expressed in CC. Twenty-six patients with histologically confirmed CC were included in this study. The expression of mucin was studied by immunohistochemistry using antibodies to MUC1, MUC1 core, MUC2, MUC3, MUC4, MUC5AC, and MUC6. There was extensive (>50%) expression of mucin, mainly MUC1 in 11/25 and MUC5AC in 12/26 cases. In the case of MUC3, 6/26 cases expressed mucin extensively, whilst only 1/26 had MUC2, MUC4, and MUC6 expression. Well-differentiated tumors significantly expressed MUC3 extensively compared to poor or moderately differentiated tumors (p=0.003). Fifteen of 25 cases had metastatic disease. MUC1 was extensively expressed in 9/15 cases with metastatic disease. In contrast, MUC1 expression was present in 2/10 cases where metastases were absent. Hilar lesions were less likely to express MUC1, but this was not statistically significant. Fifteen of 25 cases had metastatic disease. Extensive MUC3 expression was significantly associated with well-differentiated tumors, whilst there was an approaching significance between the extensive expression of MUC1 and metastasis in cholangiocarcinoma.
胆管癌（CC）是一种高度恶性的胆道上皮癌，其细胞和分子发病机制尚不清楚。腺上皮细胞的恶性转化与粘蛋白表达的改变有关。我们研究了 CC 中表达的粘蛋白类型。本研究纳入了 26 例经组织学证实的 CC 患者。通过使用针对 MUC1、MUC1 核心、MUC2、MUC3、MUC4、MUC5AC 和 MUC6 的抗体，通过免疫组织化学研究粘蛋白的表达。在 25 例中有 11 例（11/25）广泛（>50%）表达粘蛋白，主要是 MUC1，12 例（12/26）广泛表达 MUC5AC。在 MUC3 的情况下，26 例中有 6 例广泛表达粘蛋白，而仅有 1 例（1/26）表达 MUC2、MUC4 和 MUC6。与低分化或中分化肿瘤相比，高分化肿瘤显著广泛表达 MUC3（p=0.003）。25 例中有 15 例有转移病变。在有转移病变的 9/15 例中，MUC1 广泛表达。相比之下，在无转移的 10 例中，MUC1 表达存在。肝门病变不太可能表达 MUC1，但这没有统计学意义。25 例中有 15 例有转移病变。MUC3 的广泛表达与高分化肿瘤显著相关，而在胆管癌中，MUC1 的广泛表达与转移之间存在显著关联。
