Krummel Daniel Aaron Pomeranz, Nagai Kiyoshi, Oubridge Chris
F1000 Biol Rep. 2010 May 24;2:39. doi: 10.3410/B2-39.
Splicing of the precursors of eukaryotic mRNA and some non-coding RNAs is catalyzed by the 'spliceosome', which comprises five RNA-protein complexes (small nuclear ribonucleoproteins, or snRNPs) that assemble in an ordered manner onto precursor-mRNAs. Much progress has been made in determining the gross morphology of spliceosomal assembly intermediates. Recently, the first crystal structure of a spliceosomal snRNP has provided significant insight into assembly and architecture of spliceosomal snRNPs in general and the structure-function relationship of human U1 snRNP in particular.
真核生物mRNA前体和一些非编码RNA的剪接由“剪接体”催化，剪接体由五种RNA-蛋白质复合物（小核核糖核蛋白，或snRNP）组成，这些复合物以有序方式组装到前体mRNA上。在确定剪接体组装中间体的总体形态方面已经取得了很大进展。最近，剪接体snRNP的首个晶体结构为一般剪接体snRNP的组装和结构，特别是人类U1 snRNP的结构-功能关系提供了重要见解。
