Department of Biology, University of New Mexico, MSC032020, Albuquerque, NM 87131, USA.
Mar Drugs. 2010 Aug 24;8(8):2417-34. doi: 10.3390/md8082417.
For development to proceed normally, animal eggs must undergo a maturation process that ultimately depends on phosphorylations of key regulatory proteins. To analyze the kinases that mediate these phosphorylations, eggs of marine nemertean worms have been treated with pharmacological modulators of intracellular signaling pathways and subsequently probed with immunoblots employing phospho-specific antibodies. This article both reviews such analyses and compares them with those conducted on mammals, while focusing on how egg maturation in nemerteans is affected by signaling pathways involving cAMP, mitogen-activated protein kinases, Src-family kinases, protein kinase C isotypes, AMP-activated kinase, and the Cdc2 kinase of maturation-promoting factor.
为了使发育正常进行，动物卵子必须经历一个成熟过程，这个过程最终取决于关键调节蛋白的磷酸化。为了分析介导这些磷酸化的激酶，我们用细胞内信号通路的药理学调节剂处理海洋纽形动物的卵子，然后用磷酸特异性抗体进行免疫印迹探测。本文综述了这些分析，并将其与哺乳动物的分析进行了比较，同时重点介绍了参与 cAMP、丝裂原活化蛋白激酶、Src 家族激酶、蛋白激酶 C 同工型、AMP 激活的蛋白激酶和成熟促进因子的 Cdc2 激酶的信号通路如何影响纽形动物卵子的成熟。
