State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071 Wuhan, China.
Med Microbiol Immunol. 2011 Feb;200(1):61-8. doi: 10.1007/s00430-010-0175-9. Epub 2010 Oct 15.
In this study, we presented the construction of a library of expression clones for the herpes simplex virus type 1 (HSV-1) proteome and subcellular localization map of HSV-1 proteins in living cells using yellow fluorescent protein (YFP) fusion proteins. As a result, 21 proteins showed cytoplasmic or subcytoplasmic localization, 16 proteins showed nuclear or subnuclear localization, and others were present both in the nucleus and cytoplasm. Interestingly, most capsid proteins showed enriched or exclusive localization in the nucleus, and most of the envelope proteins showed cytoplasmic localization, suggesting that subcellular localization of the proteins correlated with their functions during virus replication. These results present a subcellular localization map of HSV-1 proteins in living cells, which provide useful information to further characterize the functions of these proteins.
在这项研究中，我们使用黄色荧光蛋白（YFP）融合蛋白展示了单纯疱疹病毒 1 型（HSV-1）蛋白质组的表达克隆文库的构建以及 HSV-1 蛋白在活细胞中的亚细胞定位图谱。结果，有 21 种蛋白质显示细胞质或亚细胞质定位，16 种蛋白质显示核或亚核定位，其他蛋白质则存在于细胞核和细胞质中。有趣的是，大多数衣壳蛋白显示在核内富集或特异性定位，大多数包膜蛋白显示细胞质定位，这表明蛋白质的亚细胞定位与其在病毒复制过程中的功能相关。这些结果展示了活细胞中 HSV-1 蛋白的亚细胞定位图谱，为进一步研究这些蛋白的功能提供了有用的信息。
