Jorge P A, Curti H V, Metze K
Faculdade de Ciências Médicas-UNICAMP, Campinas.
Arq Bras Cardiol. 1990 Dec;55(6):355-60.
The study of coronary flow reserve on the reperfused myocardium damaged by 90 minutes of severe ischemia.
Nine mongrel dogs of either sex, weighing 12 to 17 kg were studied. Each dog was anesthesiated with 30 mg of pentobarbital and ventilated with room air. A thoracotomy was done and the left descendent coronary artery was isolated. Aorta and right atria pressures was measured using a Siemmens-Elema transducers and mingograf recording-804 (Siemmens Instruments). Coronary blood flow was measures with a Caroline Medical Electromagnetic flowmeter, 501D. The index studied was the coronary resistance, calculated as the quotient of the diastolic aortic pressure and the diastolic blood flow. The coronary reserve was studied during reactive hyperemia produced by 10 seconds of coronary occlusion in the 5, 10, 15 and 20 minutes of reperfusion. The infarcted area was detected by TTC (triphenyltetrazolium) method.
All the hearts examined showed infarcted area. The diastolic coronary resistance during the reactive hyperemia in the 20 minutes of reperfusion was higher than the control value.
The coronary vascular reserve was decreased during reperfusion, in the myocardial infarction.
研究严重缺血90分钟后再灌注心肌的冠状动脉血流储备。
对9只体重12至17千克的杂种犬（雌雄不限）进行研究。每只犬用30毫克戊巴比妥麻醉，并用室内空气通气。进行开胸手术，分离左冠状动脉降支。使用西门子 - 埃勒玛传感器和明古格拉夫记录仪804（西门子仪器）测量主动脉和右心房压力。用卡罗琳医用电磁流量计501D测量冠状动脉血流量。所研究的指标是冠状动脉阻力，计算方法为舒张期主动脉压与舒张期血流量的商。在再灌注的第5、10、15和20分钟，通过冠状动脉闭塞10秒产生反应性充血期间研究冠状动脉储备。用TTC（三苯基四氮唑）法检测梗死面积。
所有检查的心脏均显示有梗死面积。再灌注20分钟时反应性充血期间的舒张期冠状动脉阻力高于对照值。
在心肌梗死再灌注期间，冠状动脉血管储备降低。
