Department of Environmental Science, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, South Korea.
Chemosphere. 2011 Jan;82(3):451-9. doi: 10.1016/j.chemosphere.2010.09.055. Epub 2010 Oct 16.
We characterized fluorescent silica nanoparticles (FNPs), which had been applied in many biological systems, in fish embryo rearing media (ERM) solution and evaluated the potential toxicity to the early development of Oryzias latipes embryos. Distribution of FNPs in embryos and larvae of O. latipes was studied by fluorescent and confocal laser scanning microscopic studies. Embryos exposed to three different concentrations of FNPs in stirred or sonicated ERM solutions were observed up to 2d after hatching. FNPs had a negligible effect on the hatchability of O. latipes embryos; however, compared to controls, more than 30% of eggs were abnormal in 10 and 50 mg FN P L(-1) solutions. We found that the toxic effect was increased in sonicated FNP solution, which seems to be related with the dissolution of FNPs in ERM solutions that could be accelerated by sonication. Further study found that the CaCl2 included in ERM solution might enhance the dissolution of the FNPs and the silicate ion released from FNPs partially contributed to larval toxicity. This study showed that some nanoparticles may not be stable in biological fluids even if they are stable in water. Dissolution factors such as sonication and cellular components should be considered in biological application of nanoparticles.
我们对已应用于许多生物系统的荧光硅纳米颗粒（FNPs）进行了特性描述，研究了其在鱼类胚胎养殖介质（ERM）溶液中的潜在毒性及其对 Oryzias latipes 胚胎早期发育的影响。通过荧光和共聚焦激光扫描显微镜研究了 FNPs 在 O. latipes 胚胎和幼虫中的分布。在搅拌或超声 ERM 溶液中，将胚胎暴露于三种不同浓度的 FNPs 中，直到孵化后 2 天进行观察。FNPs 对 O. latipes 胚胎的孵化率几乎没有影响；然而，与对照组相比，在 10 和 50 mg FNP L(-1)溶液中，超过 30%的卵异常。我们发现，超声 FNPs 溶液的毒性作用增强，这似乎与 FNPs 在 ERM 溶液中的溶解有关，超声可能加速了 FNPs 的溶解。进一步的研究发现，ERM 溶液中包含的 CaCl2 可能会增强 FNPs 的溶解，而 FNPs 释放的硅酸盐离子部分导致了幼虫毒性。本研究表明，即使在水中稳定，某些纳米颗粒在生物流体中也可能不稳定。在纳米颗粒的生物应用中，应考虑溶解因素（如超声和细胞成分）。
