Worsfold Water Quality Centre, Trent University, Peterborough, Ontario, Canada.
Chemosphere. 2011 Feb;82(7):1002-9. doi: 10.1016/j.chemosphere.2010.10.068. Epub 2010 Nov 11.
In this study, fertilized Japanese medaka (Oryzias latipes) embryos were exposed from fertilization to 5 d post-hatch using static non-renewal assays to aqueous suspensions of titanium dioxide nanoparticles (nTiO₂) ranging in nominal concentrations between 0 and 14 μg mL⁻¹. The average size of the nTiO₂ in the stock solution before addition to the test treatments was 87 nm (±14 nm). TiO₂ materials accumulated in a concentration dependent manner on the chorionic filaments of developing medaka embryos with evidence of pericardial edema occurring during embryo development. However, no significant (p > 0.05) increases in mortality relative to control treatments were observed for the nTiO₂ exposed embryos. A concentration dependent increase in cumulative percent hatch was observed at 11 d, indicating that exposure to increasing concentrations of nTiO₂ resulted in the premature hatch of medaka embryos. Post-hatch, a significant proportion of sac fry from the nTiO₂ exposure groups exhibited moribund swimming behavior and these individuals also experienced greater mortality at 15 d post-hatch. Combined, these results demonstrate that exposure to nTiO₂ can impact the development of early life stages of fish.
在这项研究中，受精后的日本青鳉（Oryzias latipes）胚胎从受精到孵化后 5 天，通过静态非更新试验，暴露于浓度范围在 0 至 14μg mL⁻¹ 的 TiO₂纳米颗粒（nTiO₂）的水溶液中。加入试验处理之前，储备溶液中 nTiO₂的平均粒径为 87nm（±14nm）。TiO₂材料以浓度依赖的方式在发育中的青鳉胚胎的绒毛膜丝上积累，胚胎发育过程中出现心包水肿的证据。然而，与对照处理相比，nTiO₂暴露的胚胎死亡率没有显著增加（p＞0.05）。在 11 天观察到累积孵化百分比呈浓度依赖性增加，表明暴露于浓度增加的 nTiO₂导致青鳉胚胎提前孵化。孵化后，来自 nTiO₂暴露组的大量幼鱼表现出濒死的游动行为，这些个体在孵化后 15 天的死亡率也更高。综合这些结果表明，暴露于 nTiO₂会影响鱼类早期生命阶段的发育。
