Mirzadeh S, Brechbiel M W, Atcher R W, Gansow O A
Inorganic and Radioimmune Chemistry Section, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Bioconjug Chem. 1990 Jan-Feb;1(1):59-65. doi: 10.1021/bc00001a007.
A study was made of the covalent attachment of the bifunctional 2-(4-isothiocyanatobenzyl)diethylenetriaminetetraacetic acid family of chelate ligands to proteins for the purpose of labeling monoclonal antibodies with radiometals. The parameters and the chemical variables examined included pH, reaction period, temperature, and ligand and protein concentrations. It is shown that these variables, with the exception of protein concentration, have significant effects on the rate of protein conjugation. Conjugation of three monoclonal antibodies and human IgG under identical conditions showed only 17% variation. Finally, the effect of the concentration of conjugated IgG on radiolabeling yield was studied.
为了用放射性金属标记单克隆抗体，对双功能螯合配体2-(4-异硫氰酸苄基)二乙烯三胺四乙酸家族与蛋白质的共价连接进行了研究。所考察的参数和化学变量包括pH值、反应时间、温度以及配体和蛋白质浓度。结果表明，除蛋白质浓度外，这些变量对蛋白质偶联速率有显著影响。在相同条件下对三种单克隆抗体和人IgG进行偶联，结果显示差异仅为17%。最后，研究了偶联IgG浓度对放射性标记产率的影响。
