Department of Chemistry, University of Cincinnati, 404 Crosley Tower, Cincinnati, OH 45221, USA.
Anal Bioanal Chem. 2011 Jan;399(2):701-6. doi: 10.1007/s00216-010-4272-9. Epub 2010 Oct 16.
Heparin, a heterogeneous polysaccharide, is used extensively as an anticoagulant. Recently, however, tainted heparin was associated with acute reactions that lead to numerous deaths. Extensive investigations ultimately showed oversulfated chondroitin sulfate, a semi-synthetic polysaccharide, to be present in the contaminated samples. These events highlighted the need for new, convenient heparin-screening methods capable of rapidly determining sample purity. In this work, we report the use of circular dichroism spectroscopy to analyze heparin samples for the presence of heparin-like adulterants (e.g., chondroitin sulfate A, dermatan sulfate, and oversulfated chondroitin sulfate) in a simple and straightforward manner. This strategy exploits the subtle differences in the optical properties of each polymer; these differences result from structural dissimilarities. To the best of our knowledge, the findings presented here are the first report of heparin purity screening using traditional spectropolarimetry techniques.
肝素是一种不均一的多糖，被广泛用作抗凝剂。然而，最近受污染的肝素与导致许多人死亡的急性反应有关。广泛的调查最终表明，半合成多糖硫酸皮肤素存在于受污染的样品中。这些事件突出表明需要新的、方便的肝素筛选方法，以便能够快速确定样品的纯度。在这项工作中，我们报告了使用圆二色性光谱法以简单直接的方式分析肝素样品中是否存在肝素类似的掺杂物（例如，硫酸软骨素 A、硫酸皮肤素和过度硫酸化的软骨素）。该策略利用了每种聚合物光学性质的细微差异；这些差异源于结构的不同。据我们所知，这里提出的发现是首次使用传统旋光光谱技术进行肝素纯度筛选的报告。
