Periodontal Research Group, School of Dentistry, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.
J Clin Periodontol. 2010 Dec;37(12):1039-48. doi: 10.1111/j.1600-051X.2010.01622.x. Epub 2010 Oct 18.
There is an inverse relationship between pocket depth and pocket oxygen tension with deep pockets being associated with anaerobic bacteria. However, little is known about how the host tissues respond to bacteria under differing oxygen tensions within the periodontal pocket.
To investigate the effect of different oxygen tensions upon nuclear factor-kappa B (NF-κB) activation and the inflammatory cytokine response of oral epithelial cells when exposed to nine species of oral bacteria.
H400 oral epithelial cells were equilibrated at 2%, 10% or 21% oxygen. Cells were stimulated with heat-killed oral bacteria at multiplicity of infection 10:1, Escherichia coli lipopolysaccharide (15 μg/ml) or vehicle control. Interleukin-8 (IL-8) and tumour necrosis factor-alpha (TNF-α) levels were measured by enzyme-linked immunosorbent assay and NF-κB activation was measured by reporter vector or by immunohistochemical analysis.
Tannerella forsythensis, Porphyromonas gingivalis and Prevotella intermedia elicited the greatest epithelial NF-κB activation and cytokine responses. An oxygen-tension-dependent trend in cytokine production was observed with the highest IL-8 and TNF-α production observed at 2% oxygen and lowest at 21% oxygen.
These data demonstrate a greater pro-inflammatory host response and cell signalling response to bacteria present in more anaerobic conditions, and hypersensitivity of epithelial cells to pro-inflammatory stimuli at 2% oxygen, which may have implications for disease pathogenesis and/or therapy.
牙周袋内的口袋深度与口袋氧张力呈反比关系，深口袋与厌氧菌有关。然而，对于宿主组织在牙周袋内不同氧张力下如何应对细菌，我们知之甚少。
研究不同氧张力对核因子-κB（NF-κB）激活和口腔上皮细胞炎症细胞因子反应的影响，当暴露于九种口腔细菌时。
将 H400 口腔上皮细胞在 2%、10%或 21%的氧气下平衡。用热灭活的口腔细菌以感染复数 10:1、大肠杆菌脂多糖（15μg/ml）或载体对照刺激细胞。通过酶联免疫吸附试验测量白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）水平，通过报告载体或免疫组织化学分析测量 NF-κB 激活。
福赛坦纳氏菌、牙龈卟啉单胞菌和中间普氏菌引起上皮 NF-κB 激活和细胞因子反应最强。细胞因子产生呈氧张力依赖性趋势，在 2%氧时观察到最高的 IL-8 和 TNF-α产生，在 21%氧时最低。
这些数据表明，在更厌氧条件下存在的细菌会引起更强的促炎宿主反应和细胞信号反应，以及上皮细胞对 2%氧促炎刺激的超敏反应，这可能对疾病发病机制和/或治疗有影响。
